Methanobrevibacter ruminantium as an Indicator of Domesticated-Ruminant Fecal Pollution in Surface Waters

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Methanobrevibacter ruminantium as an indicator of domesticated-ruminant fecal pollution in surface waters.

A PCR-based assay (Mrnif) targeting the nifH gene of Methanobrevibacter ruminantium was developed to detect fecal pollution from domesticated ruminants in environmental water samples. The assay produced the expected amplification product only when the reaction mixture contained DNA extracted from M. ruminantium culture, bovine (80%), sheep (100%), and goat (75%) feces, and water samples from a ...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2007

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.00911-07